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The fluorometric method is one of the official methods employed to determine the moisture content of various materials. It is a technique that utilizes the fluorescence emitted by a fluorescent dye when exposed to a specific wavelength of light. The intensity of the fluorescence is directly proportional to the moisture content present in the sample.
Principle
- A representative sample of the material is collected and weighed.
- The sample is mixed with a fluorescent dye that selectively absorbs moisture.
- The mixture is exposed to ultraviolet light, causing the dye to emit fluorescence.
- The intensity of the fluorescence is measured using a fluorometer.
- The moisture content is calculated based on a calibration curve that correlates the fluorescence intensity with known moisture levels.
Advantages
- Highly sensitive and accurate.
- Can be used for a wide range of materials.
- Non-destructive technique.
Disadvantages
- Requires specialized equipment and expertise.
- Calibration is crucial to ensure accuracy.
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